Can you describe a time when you had to adapt your experimental protocols to unexpected challenges?

Gene Therapy Scientist Interview Questions

Sample answer to the question

There was a time when I was conducting experiments to develop a gene delivery system. I encountered an unexpected challenge when the cells I was working with were not responding as expected to the gene editing tool I was using. I needed to adapt my experimental protocols to address this issue. I researched alternative gene editing tools and decided to try a different approach using a different tool. I modified my experimental protocols accordingly and repeated the experiments. Fortunately, the new approach worked, and I was able to successfully edit the genes of the cells. This experience taught me the importance of being flexible and adaptable in the face of unexpected challenges in the lab.

A more solid answer

During my work on developing a gene delivery system, I faced a challenge when the cells I was working with showed resistance to the gene editing tool CRISPR-Cas9. This was unexpected as previous studies had indicated its effectiveness in similar cell lines. To address this challenge, I decided to explore alternative gene editing tools. Through extensive literature review and discussions with colleagues, I identified a different gene editing tool that was known to have higher efficiency in the specific cell line. I modified my experimental protocols to incorporate this alternative tool and performed the experiments again. The results were promising, indicating successful gene editing. This experience taught me the importance of staying updated with the latest research and being adaptable in the face of unexpected challenges.

Why this is a more solid answer:

The solid answer provides a more detailed description of the unexpected challenge faced by the candidate and how they adapted their experimental protocols. It specifically mentions the evaluation areas and skills mentioned in the job description, such as molecular biology, laboratory techniques, gene editing tools, problem-solving skills, and the ability to work effectively in independent and team environments. However, it could be further improved by providing specific examples of the alternative gene editing tool and discussing how the candidate effectively communicated and collaborated with colleagues.

An exceptional answer

During my work on developing a gene delivery system, I encountered an unexpected challenge when the cells I was working with showed resistance to CRISPR-Cas9, the gene editing tool I was using. This was surprising as previous studies had demonstrated its efficacy in similar cell lines. To address this challenge, I took a proactive approach. I initiated discussions with senior scientists and colleagues, seeking their insights and recommendations. Through these discussions, I learned about a recently published study that highlighted an alternative gene editing tool, TALENs, which demonstrated high efficiency in the specific cell line I was working with. I thoroughly reviewed the literature and experimental protocols associated with TALENs and devised a plan to modify my experimental protocols accordingly. I coordinated with my supervisor and colleagues to obtain the necessary reagents and resources for implementing this alternative approach. The modified protocols involved optimizing the TALENs design, transfection conditions, and post-transfection analysis techniques. The experiments were repeated, and the results were highly encouraging, with successful gene editing observed in the cells. This experience not only demonstrated my ability to adapt experimental protocols to unexpected challenges but also showcased my proactive nature in seeking solutions collaboratively and staying informed about the latest advancements in the field. It further strengthened my skills in molecular biology, laboratory techniques, gene editing tools, problem-solving, and effective communication and collaboration within a team.

Why this is an exceptional answer:

The exceptional answer provides a comprehensive and detailed account of the unexpected challenge faced by the candidate, their proactive approach in seeking solutions, and their effective communication and collaboration with senior scientists and colleagues. It demonstrates the candidate's ability to adapt experimental protocols and showcases their strong skills in molecular biology, laboratory techniques, gene editing tools, problem-solving, and working effectively in independent and team environments. The answer also highlights the candidate's ability to stay updated with the latest research and advancements in the field. This answer exceeds the basic and solid answers by providing specific examples, in-depth explanations, and showcasing the candidate's exceptional performance.

How to prepare for this question

Familiarize yourself with different gene editing tools and their applications in gene therapy research. Stay updated with recent advancements in the field.
Develop a strong understanding of molecular biology techniques and laboratory protocols commonly used in gene therapy research, including cloning, PCR, and plasmid construction.
Prepare examples from your past experiences where you encountered unexpected challenges in the lab and successfully adapted your experimental protocols.
Highlight your problem-solving skills, ability to work independently and in a team, and effective communication and collaboration skills in your responses.
Be prepared to discuss how you stay organized and manage your time effectively in a laboratory setting, particularly when facing unexpected challenges.

What interviewers are evaluating

Molecular biology
Laboratory techniques
Gene editing tools (e.g., CRISPR-Cas9)
Problem-solving skills
Ability to work effectively in independent and team environments