What is your approach to problem-solving in the laboratory?

Gene Therapy Scientist Interview Questions

Sample answer to the question

My approach to problem-solving in the laboratory involves a systematic and analytical mindset. I start by thoroughly understanding the problem at hand and establishing clear objectives. Then, I gather all the relevant information and data through research and experiments. I analyze the data using appropriate statistical methods and tools to identify patterns and trends. Once I have a comprehensive understanding of the problem, I brainstorm potential solutions and evaluate their feasibility and impact. I prioritize the most viable solutions and develop a step-by-step plan to implement them. Throughout the process, I collaborate with my team members and seek their input to ensure a thorough and well-rounded approach. I also keep track of the progress and make necessary adjustments along the way. Finally, I evaluate the outcome of the solution and learn from the experience to improve my problem-solving skills for future challenges.

A more solid answer

In the laboratory, I approach problem-solving with a systematic and analytical mindset. For instance, during my internship at a biotech company, we encountered an issue with low transfection efficiency in our gene delivery system. To address this, I conducted a literature review to understand the possible causes and solutions. I designed a series of experiments to optimize the transfection conditions, including adjusting the ratio of DNA and lipofectamine, optimizing the incubation time, and testing different cell lines. I carefully documented all the experimental details and collected data, which I analyzed using statistical software. Based on the results, I identified the most effective parameter combinations and successfully improved the transfection efficiency by 50%. This experience highlighted the importance of research, data analysis, and iterative problem-solving in the laboratory.

Why this is a more solid answer:

The solid answer expands on the basic answer by providing a specific example from the candidate's past experience that demonstrates their analytical and problem-solving skills in a laboratory setting. The candidate describes the approach taken, the steps followed, and the outcome achieved. However, it could still be improved by providing more insights into collaboration skills and research skills, as mentioned in the evaluation areas.

An exceptional answer

In the laboratory, my approach to problem-solving is multifaceted and highly effective. Let me illustrate this with an example from my previous role as a research assistant at a renowned gene therapy lab. We faced a challenge in optimizing the expression of our gene of interest in a certain cell line. To tackle this, I first collaborated with my supervisor and fellow researchers to analyze previous studies and understand the underlying factors affecting gene expression. We then devised a comprehensive experimental plan to test different promoter sequences, optimize culture conditions, and utilize gene editing tools like CRISPR-Cas9. I showcased my research skills by expertly performing cloning, PCR, and transfection assays. Through careful data analysis and meticulous documentation, I identified a novel promoter sequence that significantly enhanced gene expression by 3-fold compared to the previous method. This achievement not only demonstrated my strong analytical and problem-solving skills but also showcased my ability to collaborate effectively with a multidisciplinary team and stay up-to-date with the latest gene editing technologies. By applying this holistic approach to problem-solving, I constantly strive for breakthrough solutions in the laboratory.

Why this is an exceptional answer:

The exceptional answer goes beyond the solid answer by presenting a detailed and impressive example from the candidate's previous role. It showcases their strong research skills, collaboration abilities, and their commitment to staying up-to-date with the latest technologies in gene therapy. The candidate effectively addresses all the evaluation areas mentioned in the job description. The answer also displays a high level of enthusiasm and dedication to problem-solving in the laboratory.

How to prepare for this question

Familiarize yourself with various molecular biology techniques such as PCR, cloning, and transfection, as they are commonly used in gene therapy research.
Stay updated with the latest advancements in gene therapy and gene editing tools like CRISPR-Cas9.
Develop your analytical and data analysis skills by practicing statistical methods commonly used in scientific research.
Highlight any past experiences where you successfully solved complex problems in a laboratory setting, emphasizing your approach, collaboration, and the outcomes achieved.
Practice explaining your problem-solving process in a clear and concise manner, focusing on the steps you take to analyze the problem, gather information, and develop solutions.
Highlight your ability to adapt and learn from failures or challenges faced during problem-solving.

What interviewers are evaluating

Analytical skills
Problem-solving ability
Collaboration skills
Research skills